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Pisum-sativum epicotyls inoculated with agrobacterium-rhizogenes agropine strains harboring various transfer DNA fragments : morphology, histology and endogenous indole-3-acetic-acid and indole-3-acetamide content
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| Abstract |
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| In the present paper we correlate endogenous IAA and IAM levels with morphological and histological changes of decapitated Pisum sativum epicotyls after inoculation with pA-303,d16Agrobacterium derived strains. Callus and subsequent root formation in the infected region are correlated with endogenous IAA accumulation as a result of expression of the TR aux genes, concomitant with IAM accumulation. Irrespective of the bacterial strain analyzed IAA synthesis by the bacteria themselves was found to be constitutive and therefore not related to root proliferation. Inoculation with strains harbouring the aux genes (TR or TR + TL) induced callus formation, root initiation and histological changes such as tracheid differentiation and development of cribrovascular nodules surrounded by cambial-like layers. The presence of TL largely abolished the geotropic growth of the neoformed roots. Inoculations with strains harbouring only TL DNA or the Eco15 fragment covering orf10 to 12 lead to limited histological effects and root initiation. Morphology and growth pattern of root clones obtained from inoculations with the wild type A4 pA-303,d16oculations with strains containing either TL or TR alone differed greatly and could be related to varying endogenous IAA levels. High endogenous IAA and IAM levels were found in root clones obtained after inoculation with bacterial strains carrying only TL DNA. | | |
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English
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Journal of plant physiology. - - | |
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1992
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0176-1617
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140:1(1992), p. 75-83
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A1992HZ26900015
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