Title
Affinity purification of human <tex>$\tau$</tex>-proteins and the construction of a sensitive sandwich enzyme-linked-immunosorbent-assay for human <tex>$\tau$</tex>-detection Affinity purification of human <tex>$\tau$</tex>-proteins and the construction of a sensitive sandwich enzyme-linked-immunosorbent-assay for human <tex>$\tau$</tex>-detection
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Oxford ,
Subject
Chemistry
Biology
Human medicine
Source (journal)
Journal of neurochemistry. - Oxford
Volume/pages
58(1992) :2 , p. 548-553
ISSN
0022-3042
ISI
A1992HA10700019
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Immunoaffinity chromatography with a monoclonal antibody produced against bovine tau-protein was used to purify tau-proteins from human brain. Fifty grams of brain tissue yielded approximately 2 mg of pure tau-proteins. The affinity-purified human-tau was used to produce a high-titered rabbit anti-human tau-serum. The monoclonal anti-tau antibody and the polyclonal rabbit anti-tau serum were then used to construct a sandwich enzyme-linked immunosorbent assay for detection of human tau-proteins, with a sensitivity of 1 ng/ml.
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