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Title
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Affinity purification of human -proteins and the construction of a sensitive sandwich enzyme-linked-immunosorbent-assay for human -detection
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Author
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Abstract
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| Immunoaffinity chromatography with a monoclonal antibody produced against bovine tau-protein was used to purify tau-proteins from human brain. Fifty grams of brain tissue yielded approximately 2 mg of pure tau-proteins. The affinity-purified human-tau was used to produce a high-titered rabbit anti-human tau-serum. The monoclonal anti-tau antibody and the polyclonal rabbit anti-tau serum were then used to construct a sandwich enzyme-linked immunosorbent assay for detection of human tau-proteins, with a sensitivity of 1 ng/ml. |
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Language
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English
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Source (journal)
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Journal of neurochemistry. - Oxford
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Publication
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Oxford
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1992
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ISSN
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0022-3042
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DOI
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10.1111/J.1471-4159.1992.TB09754.X
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Volume/pages
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58
:2
(1992)
, p. 548-553
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ISI
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A1992HA10700019
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Full text (Publisher's DOI)
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