Publication
Title
Affinity purification of human -proteins and the construction of a sensitive sandwich enzyme-linked-immunosorbent-assay for human -detection
Author
Abstract
Immunoaffinity chromatography with a monoclonal antibody produced against bovine tau-protein was used to purify tau-proteins from human brain. Fifty grams of brain tissue yielded approximately 2 mg of pure tau-proteins. The affinity-purified human-tau was used to produce a high-titered rabbit anti-human tau-serum. The monoclonal anti-tau antibody and the polyclonal rabbit anti-tau serum were then used to construct a sandwich enzyme-linked immunosorbent assay for detection of human tau-proteins, with a sensitivity of 1 ng/ml.
Language
English
Source (journal)
Journal of neurochemistry. - Oxford
Publication
Oxford : 1992
ISSN
0022-3042
DOI
10.1111/J.1471-4159.1992.TB09754.X
Volume/pages
58 :2 (1992) , p. 548-553
ISI
A1992HA10700019
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 19.07.2012
Last edited 06.12.2021
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