| Title |
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Functional analysis of cyclin-dependent kinase inhibitors of **Arabidopsis**
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| Author |
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| Abstract |
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| Cyclin-dependent kinase inhibitors, such as the mammalian p27
Kip1
protein, regulate correct cell cycle progression and
the integration of developmental signals with the core cell cycle machinery. These inhibitors have been described in
plants, but their function remains unresolved. We have isolated seven genes from Arabidopsis that encode proteins
with distant sequence homology with p27
Kip1
, designated Kip-related proteins (KRPs). The KRPs were characterized by
their domain organization and transcript profiles. With the exception of KRP5, all presented the same cyclin-dependent
kinase binding specificity. When overproduced, KRP2 dramatically inhibited cell cycle progression in leaf primordia
cells without affecting the temporal pattern of cell division and differentiation. Mature transgenic leaves were serrated
and consisted of enlarged cells. Although the ploidy levels in young leaves were unaffected, endoreduplication was
suppressed in older leaves. We conclude that KRP2 exerts a plant growth inhibitory activity by reducing cell proliferation
in leaves, but, in contrast to its mammalian counterparts, it may not control the timing of cell cycle exit and differentiation. | | |
| Language |
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English
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| Source (journal) |
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The plant cell. - Rockville, Md | |
| Publication |
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Rockville, Md : 2001
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| ISSN |
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1040-4651
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| Volume/pages |
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13:7(2001), p. 1653-1668
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| ISI |
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000170061400013
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| Full text (Publisher's DOI) |
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