Title
Functional analysis of cyclin-dependent kinase inhibitors of **Arabidopsis** Functional analysis of cyclin-dependent kinase inhibitors of **Arabidopsis**
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Rockville, Md ,
Subject
Biology
Source (journal)
The plant cell. - Rockville, Md
Volume/pages
13(2001) :7 , p. 1653-1668
ISSN
1040-4651
ISI
000170061400013
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Cyclin-dependent kinase inhibitors, such as the mammalian p27 Kip1 protein, regulate correct cell cycle progression and the integration of developmental signals with the core cell cycle machinery. These inhibitors have been described in plants, but their function remains unresolved. We have isolated seven genes from Arabidopsis that encode proteins with distant sequence homology with p27 Kip1 , designated Kip-related proteins (KRPs). The KRPs were characterized by their domain organization and transcript profiles. With the exception of KRP5, all presented the same cyclin-dependent kinase binding specificity. When overproduced, KRP2 dramatically inhibited cell cycle progression in leaf primordia cells without affecting the temporal pattern of cell division and differentiation. Mature transgenic leaves were serrated and consisted of enlarged cells. Although the ploidy levels in young leaves were unaffected, endoreduplication was suppressed in older leaves. We conclude that KRP2 exerts a plant growth inhibitory activity by reducing cell proliferation in leaves, but, in contrast to its mammalian counterparts, it may not control the timing of cell cycle exit and differentiation.
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