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Title
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Human plasmacytoid dendritic cells are equipped with antigen presenting- and tumoricidal-capacities
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Author
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Abstract
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| Human plasmacytoid dendritic cells (pDCs) represent a highly specialized naturally occuring dendritic cell subset and are the main producers of type I interferons (IFN) in response to viral infections. We show that human pDCs activated by the preventive vaccine FSME specifically upregulate CD56 on their surface, a marker which was thought to be specific for NK cells and associated with cytolytic effector functions. We observed that FSME-activated pDCs specifically lysed NK target cells and expressed cytotoxic molecules such as TRAIL and granzyme B. Elevated levels of these molecules coincided with the expression of CD56, indicative for skewing human pDCs towards an interferon producing killer DC subset. Detailed phenotypical and functional analysis revealed that pDCs attained a mature phenotype, secreted pro-inflammatory cytokines and had the capacity to present antigens and stimulate T cells. We here report on the generation of CD56+ human interferon producing killer plasmacytoid DCs with the capacity to present antigens. These findings aid in deciphering the role for pDCs in anti-tumor immunity and present a promising prospect of developing anti-tumor therapy using pDCs. |
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Language
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English
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Source (journal)
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Blood / American Society of Hematology. - New York, N.Y.
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Publication
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New York, N.Y.
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2012
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ISSN
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0006-4971
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DOI
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10.1182/BLOOD-2012-06-435941
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Volume/pages
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120
:19
(2012)
, p. 3936-3944
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ISI
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000311629600015
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Full text (Publisher's DOI)
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