Title
Rapid isolation and identification of minor natural products by LCMS, LCSPENMR and ECD : isoflavanones, biflavanones and bisdihydrocoumarins from **Ormocarpum kirkii** Rapid isolation and identification of minor natural products by LCMS, LCSPENMR and ECD : isoflavanones, biflavanones and bisdihydrocoumarins from **Ormocarpum kirkii**
Author
Faculty/Department
Faculty of Sciences. Chemistry
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Publication type
article
Publication
Oxford ,
Subject
Chemistry
Biology
Pharmacology. Therapy
Source (journal)
Phytochemistry: an international journal of plant biochemistry. - Oxford
Volume/pages
79(2012) , p. 121-128
ISSN
0031-9422
ISI
000307031800012
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The combination of the hyphenated techniques LCMS and LCSPENMR constitutes a powerful platform for the rapid isolation and identification of minor components from natural sources. Electronic circular dichroism (ECD) is a useful tool to determine the absolute configuration of small quantities of chiral molecules. In order to search for minor constituents present in an Ormocarpum kirkii extract, these techniques were applied for the separation and structure elucidation of a series of isoflavanones, biflavanones and biscoumarins. After optimization of chromatographic conditions and subsequent isolation, MS and 1D and 2D NMR data were collected. Experimental and calculated ECD spectra were used in conjunction with NMR data to confirm the absolute configuration of these compounds. Eight compounds were identified for the first time and six have been previously reported. The present approach offers a strategy for accelerating research on natural products.
E-info
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