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Title
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Interleukin-12p70 expression by dendritic cells of HIV-1-infected patients fails to stimulate **gag**-specific immune responses
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Author
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Abstract
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| A variety of immune-based therapies has been developed in order to boost or induce protective CD8(+) T cell responses in order to control HIV replication. Since dendritic cells (DCs) are professional antigen-presenting cells (APCs) with the unique capability to stimulate naive T cells into effector T cells, their use for the induction of HIV-specific immune responses has been studied intensively. In the present study we investigated whether modulation of the activation state of DCs electroporated with consensus codon-optimized HxB2 gag mRNA enhances their capacity to induce HIV gag-specific T cell responses. To this end, mature DCs were (i) co-electroporated with mRNA encoding interleukin (IL)-12p70 mRNA, or (ii) activated with a cytokine cocktail consisting of R848 and interferon (IFN)-gamma. Our results confirm the ability of HxB2 gag-expressing DCs to expand functional HIV-specific CD8(+) T cells. However, although most of the patients had detectable gag-specific CD8(+) T cell responses, no significant differences in the level of expansion of functional CD8(+) T cells could be demonstrated when comparing conventional or immune-modulated DCs expressing IL-12p70. This result which goes against expectation may lead to a re-evaluation of the need for IL-12 expression by DCs in order to improve T-cell responses in HIV-1-infected individuals. |
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Language
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English
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Source (journal)
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Clinical and developmental immunology. - -
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Publication
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Taylor
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2012
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ISSN
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1740-2522
1740-2530
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DOI
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10.1155/2012/184979
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Volume/pages
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(2012)
, 11 p.
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Article Reference
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184979
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ISI
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000307589200001
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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Full text (open access)
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