Title
Towards a nanoscale view of lactic acid bacteria Towards a nanoscale view of lactic acid bacteria
Author
Faculty/Department
Faculty of Sciences. Bioscience Engineering
Publication type
article
Publication
Oxford ,
Subject
Chemistry
Source (journal)
Micron. - Oxford
Volume/pages
43(2012) :12 , p. 1323-1330
ISSN
0968-4328
ISI
000308680900016
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Probiotic bacteria have a strong potential in biomedicine owing to their ability to induce various beneficial health effects. Bacterial cell surface constituents play a key role in establishing tight interactions between probiotics and their host. Yet, little is known about the spatial organization and biophysical properties of the individual molecules. In this paper, we discuss how we have been using atomic force microscopy imaging and force spectroscopy to probe the nanoscale surface properties of Gram-positive lactic acid bacteria, with an emphasis on probiotic strains. Topographic imaging has enabled us to visualize bacterial cell surface structures (peptidoglycan, teichoic acids, pili, polysaccharides) under physiological conditions and with unprecedented resolution. In parallel, single-molecule force spectroscopy has been used to localize and force probe single cell surface constituents, providing novel insights into their spatial distribution and molecular elasticity. (C) 2012 Elsevier Ltd. All rights reserved.
E-info
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