Title
Accurate and rapid species typing from cutaneous and mucocutaneous leishmaniasis lesions of the New World Accurate and rapid species typing from cutaneous and mucocutaneous leishmaniasis lesions of the New World
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Amsterdam ,
Subject
Biology
Human medicine
Source (journal)
Diagnostic microbiology and infectious disease. - Amsterdam
Volume/pages
74(2012) :2 , p. 142-150
ISSN
0732-8893
ISI
000309086100008
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The heat-shock protein 70 gene (hsp70) has been exploited for Leishmania species identification in the Old and New World, using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism analysis. Three new Leishmania-specific hsp70 PCRs were recently described, and we applied 2 of these on 89 clinical samples from a total of 73 Peruvian patients with either cutaneous or mucocutaneous leishmaniasis. The new PCRs on average showed a 2- to 3-fold improved sensitivity in the tested sample types (lesion biopsies, aspirates, and scrapings), for both genus detection and species typing, and were most successful in biopsies. Leishmania braziliensis, L. peruviana, and L. guyanensis were encountered. About one third of the L braziliensis parasites contained 2 hsp70 alleles. This study is a paradigm for the implementation of a globally applicable upgraded tool for the identification of Leishmania directly on human specimens from cutaneous and mucocutaneous lesions in the New World. (C) 2012 Elsevier Inc. All rights reserved.
E-info
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https://repository.uantwerpen.be/docman/iruaauth/0850ad/e1e10332.pdf
Handle