Title
AUX/LAX genes encode a family of auxin influx transporters that perform distinct functions during Arabidopsis developmentAUX/LAX genes encode a family of auxin influx transporters that perform distinct functions during Arabidopsis development
Author
Faculty/Department
Faculty of Sciences. Biology
Research group
Integrated Molecular Plant Physiology Research (IMPRES)
Molecular Plant Physiology and Biotechnology
Publication type
article
Publication
Rockville, Md,
Subject
Chemistry
Biology
Human medicine
Source (journal)
The plant cell. - Rockville, Md
Volume/pages
24(2012):7, p. 2874-2885
ISSN
1040-4651
ISI
000308352800015
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Auxin transport, which is mediated by specialized influx and efflux carriers, plays a major role in many aspects of plant growth and development. AUXIN1 (AUX1) has been demonstrated to encode a high-affinity auxin influx carrier. In Arabidopsis thaliana, AUX1 belongs to a small multigene family comprising four highly conserved genes (i.e., AUX1 and LIKE AUX1 [LAX] genes LAX1, LAX2, and LAX3). We report that all four members of this AUX/LAX family display auxin uptake functions. Despite the conservation of their biochemical function, AUX1, LAX1, and LAX3 have been described to regulate distinct auxin-dependent developmental processes. Here, we report that LAX2 regulates vascular patterning in cotyledons. We also describe how regulatory and coding sequences of AUX/LAX genes have undergone subfunctionalization based on their distinct patterns of spatial expression and the inability of LAX sequences to rescue aux1 mutant phenotypes, respectively. Despite their high sequence similarity at the protein level, transgenic studies reveal that LAX proteins are not correctly targeted in the AUX1 expression domain. Domain swapping studies suggest that the N-terminal half of AUX1 is essential for correct LAX localization. We conclude that Arabidopsis AUX/LAX genes encode a family of auxin influx transporters that perform distinct developmental functions and have evolved distinct regulatory mechanisms.
E-info
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