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Title
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Marked difference in the electronic structure of cyanide-ligated ferric protoglobins and myoglobin due to heme ruffling
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Author
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Abstract
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| Electron paramagnetic resonance experiments reveal a significant difference between the principal g values (and hence, ligand-field parameters) of the ferric cyanide-ligated form of different variants of the protoglobin of Methanosarcina acetivorans (MaPgb) and of horse heart myoglobin (hhMb). The largest principal g value of the ferric cyanide-ligated MaPgb variants is found to be significantly lower than for any of the other globins reported so far. This is at least partially caused by the strong heme distortions as proven by the determination of the hyperfine interaction of the heme nitrogens and mesoprotons. Furthermore, the experiments confirm recent theoretical predictions [Forti, F.; Boechi, L., Bikiel, D., Marti, M.A.; Nardini, M.; Bolognesi, M.; Viappiani, C.; Estrin, D.; Luque, F. J. J. Phys. Chem. B 2011, 115, 13771-13780] that Phe(G8)145 plays a crucial role in the ligand modulation in MaPgb. Finally, the influence of the N-terminal 20 amino-acid chain on the heme pocket in these protoglobins is also proven. |
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Language
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English
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Source (journal)
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Inorganic chemistry / American Chemical Society. - Easton, Pa
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Publication
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Easton, Pa
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2012
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ISSN
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0020-1669
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DOI
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10.1021/IC3007074
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Volume/pages
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51
:16
(2012)
, p. 8834-8841
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ISI
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000307606200029
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Full text (Publisher's DOI)
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