Title
High-performance liquid-chromatographic determination of native and reduced alpha-glucan oligosaccharides on reversed-phase columns using postcolumn immobilized enzyme reactorsHigh-performance liquid-chromatographic determination of native and reduced alpha-glucan oligosaccharides on reversed-phase columns using postcolumn immobilized enzyme reactors
Author
Faculty/Department
Faculty of Sciences. Chemistry
Research group
Chromatographic Organic Trace Analysis
Publication type
article
Publication
Braunschweig,
Subject
Chemistry
Biology
Source (journal)
Chromatographia. - Braunschweig
Volume/pages
37(1993):9-10, p. 511-516
ISSN
0009-5893
ISI
A1993ML83400009
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
A post-column enzyme reactor with co-immobilized glucoamylase and glucose oxidase has been used for the detection of alpha-glucans after HPLC separation of native and NaBH4-reduced oligosaccharides. Oligomers eluting from the column were cleaved into monomers which were oxidized with production of hydrogen peroxide, which was measured amperometrically at a platinum electrode. Detection of alpha-glucans proved to be highly selective and sensitive. The enzyme reactors are very stable under the conditions used. Linear calibration graphs were observed over 3 decades, and the detection limit for various oligosaccharides is into the lower ng range. Efficient separations were obtained on reversed phase columns using gradient elution with acetonitrile. Reduced oligomers were separated up to a degree of polymerization > 40. The influence of temperature, pH, reaction time and sodium borohydride concentration was evaluated for the reduction of alpha-glucans. Reduction is complete within 2 minutes at 80 degrees C and within 20 minutes at room temperature.
E-info
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1993ML83400009&DestLinkType=RelatedRecords&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1993ML83400009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1993ML83400009&DestLinkType=CitingArticles&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848
Handle