Title
|
|
|
|
Interferon- and interleukin-4 reciprocally regulate the production of monocytes macrophages and neutrophils through a direct effect on committed monopotential bone marrow progenitor cells
| |
Author
|
|
|
|
| |
Abstract
|
|
|
|
We studied the direct effects of interferon-gamma (IFN-gamma) in single cell colony assays of CD34+HLA-DR++ bone marrow progenitor cells stimulated by either granulocyte-colony-stimulating factor (G-CSF), interleukin(IL)-3, granulocyte/macrophage-colony-stimulating factor (GM-CSF), combinations of these CSF or medium conditioned by the 5637 human bladder carcinoma cell line. In this culture system IFN-gamma stimulated monocytic colonies (CFU-M) no matter which CSF or CSF combination was used to support them and inhibited granulocytic colonies (CFU-G) if they were generated in the presence of G-CSF. IL-4 antagonized the myelopoietic effects of IFN-gamma: the IFN-gamma-induced suppression of G-CSF-supported CFU-G, as well as the stimulation of CFU-M, were reversed by IL-4. In all cultures, IFN-gamma had a limited, but statistically non-significant, inhibitory effect on CFU-GM, which was not affected by the presence of IL-4. These data show that IFN-gamma and IL-4 reciprocally regulate the generation of myeloid cells involved in humoral (neutrophils) and cellular (macrophages) immune responses through a direct effect on monopotential myeloid progenitor cells. |
| |
Language
|
|
|
|
English
| |
Source (journal)
|
|
|
|
European journal of immunology. - Weinheim
| |
Publication
|
|
|
|
Weinheim
:
1993
| |
ISSN
|
|
|
|
0014-2980
| |
DOI
|
|
|
|
10.1002/EJI.1830230514
| |
Volume/pages
|
|
|
|
23
:5
(1993)
, p. 1072-1077
| |
ISI
|
|
|
|
A1993LC38100013
| |
Full text (Publisher's DOI)
|
|
|
|
| |
|