Title
Interferon-<tex>$\gamma$</tex> and interleukin-4 reciprocally regulate the production of monocytes macrophages and neutrophils through a direct effect on committed monopotential bone marrow progenitor cells Interferon-<tex>$\gamma$</tex> and interleukin-4 reciprocally regulate the production of monocytes macrophages and neutrophils through a direct effect on committed monopotential bone marrow progenitor cells
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Weinheim ,
Subject
Human medicine
Source (journal)
European journal of immunology. - Weinheim
Volume/pages
23(1993) :5 , p. 1072-1077
ISSN
0014-2980
ISI
A1993LC38100013
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
We studied the direct effects of interferon-gamma (IFN-gamma) in single cell colony assays of CD34+HLA-DR++ bone marrow progenitor cells stimulated by either granulocyte-colony-stimulating factor (G-CSF), interleukin(IL)-3, granulocyte/macrophage-colony-stimulating factor (GM-CSF), combinations of these CSF or medium conditioned by the 5637 human bladder carcinoma cell line. In this culture system IFN-gamma stimulated monocytic colonies (CFU-M) no matter which CSF or CSF combination was used to support them and inhibited granulocytic colonies (CFU-G) if they were generated in the presence of G-CSF. IL-4 antagonized the myelopoietic effects of IFN-gamma: the IFN-gamma-induced suppression of G-CSF-supported CFU-G, as well as the stimulation of CFU-M, were reversed by IL-4. In all cultures, IFN-gamma had a limited, but statistically non-significant, inhibitory effect on CFU-GM, which was not affected by the presence of IL-4. These data show that IFN-gamma and IL-4 reciprocally regulate the generation of myeloid cells involved in humoral (neutrophils) and cellular (macrophages) immune responses through a direct effect on monopotential myeloid progenitor cells.
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