Publication
Title
Comparative evaluation of the Rapid ID 32A kit system, miniaturized standard procedure and a rapid fermentation procedure for the identification of anaerobic bacteria
Author
Abstract
The RAPID ID 32A and a rapid fermentation procedure were compared with a miniaturized standard procedure for the identification of 102 isolates of anaerobic bacteria belonging to 11 genera. The miniaturized standard procedure identified all 102 isolates at the genus level and 90 at the species level. The rapid fermentation procedure failed to identify 12 and 24 isolates at the genus and species levels respectively and is unsatisfactory. The RAPID ID 32A system misidentified 4 isolates (4%) at the genus level, 3 of which could have been avoided if the results of the Gram stain, the fundamental procedure in medical microbiology, had been taken into account. The system correctly identified 89 isolates at the species level. Since it does not produce species identifications within the genera Mobiluncus and Veillonella of which 7 and 2 isolates respectively were included in the study, the percentage of correct identifications at the species level is 96.7% (90/93). The RAPID ID 32A system is about 25 times more expensive than the miniaturized standard procedure if the investment for a gas chromatograph is not taken into account.
Language
English
Source (journal)
Acta clinica Belgica. - Leuven, 1946 - 1997
Publication
Leuven : 1993
ISSN
0001-5512
DOI
10.1080/17843286.1993.11718291
Volume/pages
48 :2 (1993) , p. 81-85
ISI
A1993LE29500002
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 03.01.2013
Last edited 23.12.2024
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