Title
Identification of enteropathogenic campylobacter species by oligonucleotide probes and polymerase chain-reaction based on 16S rRNA genes Identification of enteropathogenic campylobacter species by oligonucleotide probes and polymerase chain-reaction based on 16S rRNA genes
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Stuttgart ,
Subject
Biology
Engineering sciences. Technology
Source (journal)
Systematic and applied microbiology. - Stuttgart
Volume/pages
16(1993) :1 , p. 30-36
ISSN
0723-2020
ISI
A1993LC31900007
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
On the basis of 16S ribosomal RNA (rRNA) sequences published for Campylobacter species C. jejuni, C. coli, C. fetus and C. hyointestinalis, we were able to design three oligonucleotide probes (probes 6-1, 10-1 and 18-1r) specific only for C. jejuni and C. coli. 16S rRNA genes of 60 different Campylobacter strains were amplified by the Polymerase Chain Reaction (PCR) using universally conserved primers and the amplification products were hybridized with the probes. This analysis showed that probe 6-1 hybridizes with the 16S rRNA from C. jejuni, C. coli, C. lari and C. upsaliensis; probe 10-1 hybridizes with 16S rRNA from C. jejuni, C. coli and C. lari, while probe 18-1r hybridizes with 16S rRNA from C. jejuni, C. coli, C. lari and some strains of C. upsaliensis. When the oligonucleotides 6-1 and 18-1r are used as primers in PCR amplification, and the resulting PCR product is hybridized with the internal probe 10-1, the DNA equivalent of two bacteria can be detected specifically for the group of pathogenic species C. jejuni, C. coli, and C. lari.
E-info
https://repository.uantwerpen.be/docman/iruaauth/6342df/0814889.pdf
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