Publication
Title
Identification of enteropathogenic campylobacter species by oligonucleotide probes and polymerase chain-reaction based on 16S rRNA genes
Author
Abstract
On the basis of 16S ribosomal RNA (rRNA) sequences published for Campylobacter species C. jejuni, C. coli, C. fetus and C. hyointestinalis, we were able to design three oligonucleotide probes (probes 6-1, 10-1 and 18-1r) specific only for C. jejuni and C. coli. 16S rRNA genes of 60 different Campylobacter strains were amplified by the Polymerase Chain Reaction (PCR) using universally conserved primers and the amplification products were hybridized with the probes. This analysis showed that probe 6-1 hybridizes with the 16S rRNA from C. jejuni, C. coli, C. lari and C. upsaliensis; probe 10-1 hybridizes with 16S rRNA from C. jejuni, C. coli and C. lari, while probe 18-1r hybridizes with 16S rRNA from C. jejuni, C. coli, C. lari and some strains of C. upsaliensis. When the oligonucleotides 6-1 and 18-1r are used as primers in PCR amplification, and the resulting PCR product is hybridized with the internal probe 10-1, the DNA equivalent of two bacteria can be detected specifically for the group of pathogenic species C. jejuni, C. coli, and C. lari.
Language
English
Source (journal)
Systematic and applied microbiology. - Stuttgart
Publication
Stuttgart : 1993
ISSN
0723-2020
Volume/pages
16:1(1993), p. 30-36
ISI
A1993LC31900007
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 03.01.2013
Last edited 05.11.2017
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