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Identification of enteropathogenic campylobacter species by oligonucleotide probes and polymerase chain-reaction based on 16S rRNA genes
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| Abstract |
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| On the basis of 16S ribosomal RNA (rRNA) sequences published for Campylobacter species C. jejuni, C. coli, C. fetus and C. hyointestinalis, we were able to design three oligonucleotide probes (probes 6-1, 10-1 and 18-1r) specific only for C. jejuni and C. coli. 16S rRNA genes of 60 different Campylobacter strains were amplified by the Polymerase Chain Reaction (PCR) using universally conserved primers and the amplification products were hybridized with the probes. This analysis showed that probe 6-1 hybridizes with the 16S rRNA from C. jejuni, C. coli, C. lari and C. upsaliensis; probe 10-1 hybridizes with 16S rRNA from C. jejuni, C. coli and C. lari, while probe 18-1r hybridizes with 16S rRNA from C. jejuni, C. coli, C. lari and some strains of C. upsaliensis. When the oligonucleotides 6-1 and 18-1r are used as primers in PCR amplification, and the resulting PCR product is hybridized with the internal probe 10-1, the DNA equivalent of two bacteria can be detected specifically for the group of pathogenic species C. jejuni, C. coli, and C. lari. | | |
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English
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Systematic and applied microbiology. - Stuttgart | |
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Stuttgart : 1993
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0723-2020
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16:1(1993), p. 30-36
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A1993LC31900007
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