Title Micromethod determination of N-acetyl-$\alpha$-d-glucosaminidase in human-leukocytes and study of some of its characteristicsMicromethod determination of N-acetyl-$\alpha$-d-glucosaminidase in human-leukocytes and study of some of its characteristics Author den Tandt, W.R. Scharpe, S. Faculty/Department Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy Faculty of Medicine and Health Sciences Research group Faculteit Geneeskunde Medical Biochemistry Publication type article Publication 1993Oxford, 1993 Subject Chemistry Biology Source (journal) The international journal of biochemistry. - Oxford, 1970 - 1994 Volume/pages 25(1993):2, p. 209-212 ISSN 0020-711X ISI A1993KL92000007 Carrier E Target language English (eng) Full text (Publishers DOI) Affiliation University of Antwerp Abstract 1. A very easy fluorescent micromethod is described to measure leukocyte N-acetyl-alpha-D-glucosaminidase in human leukocytes. The method presents the following advantages: possibility to measure low activity due to prolonged linearity, very moderate consumption of substrate, use of total homogenate without the need for protein removal by precipitation after the enzyme reaction, and minimal handling after the addition of the stopping reagent. 2. A number of enzyme characteristics as linearity (up to 16 hr), pH optimum (4), thermoresistance at different temperatures (up to 24 hr at room temperature) and at different pH values (no differences between 3-8) and solubilization by digitonine and Triton X-100 were deter-mined. The characteristics (acid pH optimum, binding to Concanavaline A and stability for prolonged time at 0 and 23-degrees-C) are in agreement with the known properties of most lyosomal enzymes. 3. The specific activity of the enzyme is very low and an unexplained low latency (about 30%) was observed. E-info https://repository.uantwerpen.be/docman/iruaauth/f5fb0f/9db4893.pdf http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1993KL92000007&DestLinkType=RelatedRecords&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1993KL92000007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1993KL92000007&DestLinkType=CitingArticles&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848 Handle