Publication
Title
Micromethod determination of N-acetyl-$\alpha$-d-glucosaminidase in human-leukocytes and study of some of its characteristics
Author
Abstract
 1. A very easy fluorescent micromethod is described to measure leukocyte N-acetyl-alpha-D-glucosaminidase in human leukocytes. The method presents the following advantages: possibility to measure low activity due to prolonged linearity, very moderate consumption of substrate, use of total homogenate without the need for protein removal by precipitation after the enzyme reaction, and minimal handling after the addition of the stopping reagent. 2. A number of enzyme characteristics as linearity (up to 16 hr), pH optimum (4), thermoresistance at different temperatures (up to 24 hr at room temperature) and at different pH values (no differences between 3-8) and solubilization by digitonine and Triton X-100 were deter-mined. The characteristics (acid pH optimum, binding to Concanavaline A and stability for prolonged time at 0 and 23-degrees-C) are in agreement with the known properties of most lyosomal enzymes. 3. The specific activity of the enzyme is very low and an unexplained low latency (about 30%) was observed.
Language
English
Source (journal)
The international journal of biochemistry. - Oxford, 1970 - 1994
Publication
Oxford : 1993
ISSN
0020-711X
Volume/pages
25 :2 (1993) , p. 209-212
ISI
A1993KL92000007
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
 Faculty/Department Research group Publication type Subject Affiliation Publications with a UAntwerp address