Title
Metal concentrations, sperm motility, and RNA/DNA ratio in two echinoderm species from a highly contaminated fjord (the Sorfjord, Norway) Metal concentrations, sperm motility, and RNA/DNA ratio in two echinoderm species from a highly contaminated fjord (the Sorfjord, Norway)
Author
Publication type
article
Publication
New York, N.Y. ,
Subject
Chemistry
Biology
Pharmacology. Therapy
Source (journal)
Environmental toxicology and chemistry. - New York, N.Y.
Volume/pages
27(2008) :7 , p. 1553-1560
ISSN
0730-7268
ISI
000256806700012
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
The present study evaluated the effects of field metal contamination on sperm motility and the RNA/DNA ratio in echinoderms. Populations of Asterias rubens and Echinus acutus that occur naturally along a contamination gradient of sediments by cadmium, copper, lead, and zinc in a Norwegian fjord (the Sorfjord) were studied. Sperm motility, a measure of sperm quality, was quantified using a computer-assisted sperm analysis system. The RNA/DNA ratio, a measure of protein synthesis, was assessed by a one-dye (ethidium bromide)/one-enzyme (RNase), 96-well microplate fluorometric assay. Although both species accumulate metals at high concentrations, neither sperm motility parameters in A. rubens nor the RNA/DNA ratio in both species were affected. The Sorfjord is still one of the most metal-contaminated marine sites in Europe, but even so, populations of A. rubens and E. acutus are able to endure under these conditions.
E-info
https://repository.uantwerpen.be/docman/iruaauth/ee3241/be05749.pdf
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