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Title
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14-3-3 -isoform detection distinguishes sporadic Creutzfeldt-Jakob disease from other dementias
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Author
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Abstract
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| We developed a polyclonal antiserum directed to the gamma-isoform of the human 14-3-3 protein and compared the immunoreactivity with a commercially available antibody (CG31). We analysed 14-3-3 in 253 cerebrospinal fluid samples blinded for the diagnosis by western blot and ELISA, with a commonly used polyclonal antiserum (Sc-731) and the c specific antibodies. Our patient population consisted of 52 patients with definite sporadic Creutzfeldt - Jakob disease (sCJD) and 201 patients with a different final diagnosis. We obtained similar sensitivity, ranging from 96% to 98% with all antibodies. Of all the samples that were false positive with Sc-731, 50% were negative with both gamma-isoform specific antibodies resulting in a significantly higher specificity (85% v 93%, respectively). If only sCJD and patients with dementia differing from sCJD were analysed we found that 64% of false positives were negative which also resulted in significantly increased specificity and positive predictive value. The gamma-isoform specific antibodies strongly improve the specificity of the immunoblot and might improve worldwide acceptance of the use of the 14-3-3 assay in the differential diagnosis of sCJD. |
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Language
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English
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Source (journal)
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Journal of neurology, neurosurgery and psychiatry. - London
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Publication
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London
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2005
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ISSN
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0022-3050
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DOI
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10.1136/JNNP.2003.032037
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Volume/pages
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76
:1
(2005)
, p. 100-102
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ISI
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000225777400020
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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