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14-3-3 -isoform detection distinguishes sporadic Creutzfeldt-Jakob disease from other dementias
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| Abstract |
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| We developed a polyclonal antiserum directed to the gamma-isoform of the human 14-3-3 protein and compared the immunoreactivity with a commercially available antibody (CG31). We analysed 14-3-3 in 253 cerebrospinal fluid samples blinded for the diagnosis by western blot and ELISA, with a commonly used polyclonal antiserum (Sc-731) and the c specific antibodies. Our patient population consisted of 52 patients with definite sporadic Creutzfeldt - Jakob disease (sCJD) and 201 patients with a different final diagnosis. We obtained similar sensitivity, ranging from 96% to 98% with all antibodies. Of all the samples that were false positive with Sc-731, 50% were negative with both gamma-isoform specific antibodies resulting in a significantly higher specificity (85% v 93%, respectively). If only sCJD and patients with dementia differing from sCJD were analysed we found that 64% of false positives were negative which also resulted in significantly increased specificity and positive predictive value. The gamma-isoform specific antibodies strongly improve the specificity of the immunoblot and might improve worldwide acceptance of the use of the 14-3-3 assay in the differential diagnosis of sCJD. |
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English
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| Source (journal) |
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Journal of neurology, neurosurgery and psychiatry. - London |
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| Publication |
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London : 2005
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| ISSN |
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0022-3050
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76:1(2005), p. 100-102
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000225777400020
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