Title
Culture of bovine bone marrow progenitor cells in vitro Culture of bovine bone marrow progenitor cells in vitro
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
The Hague ,
Subject
Veterinary medicine
Source (journal)
The veterinary quarterly. - The Hague
Volume/pages
23(2001) :4 , p. 170-175
ISSN
0165-2176
ISI
000173087000006
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
In vitro methylcellulose cultures of bovine bone marrow progenitor cells were developed. An existing technique described for bovine species was compared to a method for human tissue and further adapted during subsequent experiments. Bovine bone marrow samples were collected at the slaughterhouse, and mononuclear cells were separated by gradient centrifugation (1.077 g/ml specific density and 400g). The use of 3% bovine leucocyte-conditioned medium, produced by stimulation of blood lymphocytes with 4 mug/ml concanavalin A and harvested on day 4 of culture, gave better results than the use of supernatant of the human bladder carcinoma 5637, which is widely used in human bone marrow cultures. However, bovine leucocyte-conditioned medium was not added to erythroid cultures because inhibitory effects were observed. Erythroid colonies were stimulated with erythropoietin, and hemin was added to enable microscopic identification. Reduced oxygen tension was necessary to induce growth of erythroid colonies. This was not necessary for myeloid cultures. In conclusion, the results of this study show that the growth of myeloid and erythroid colonies in methylcellulose-based medium requires different culture conditions, which are different from the culture conditions for human cells.
E-info
https://repository.uantwerpen.be/docman/iruaauth/ed6bcd/e626040.pdf
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