Title
Automatic quantification of neurite outgrowth by means of image analysis Automatic quantification of neurite outgrowth by means of image analysis
Author
Faculty/Department
Faculty of Sciences. Physics
Publication type
conferenceObject
Publication
Subject
Physics
Source (journal)
PROCESSING XI
Progress in biomedical optics and imaging
Source (book)
Conference on 3-Dimensional and Multidimensional Microscopy - Image, Acquisition and Processing XI, JAN 27-29, 2004, San Jose, CA
Volume/pages
5(2004) :13 , p. 1-7
ISBN
0-8194-5232-7
ISI
000223297800001
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
A system for quantification of neurite outgrowth in in-vitro experiments is described. The system is developed for routine use in a high-throughput setting and is therefore needs fast, cheap, and robust. It relies on automated digital microscopical imaging of microtiter plates. Image analysis is applied to extract features for characterisation of neurite outgrowth. The system is tested in a dose-response experiment on PC12 cells + Taxol. The performance of the system and its ability to measure changes on neuronal morphology is studied.
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