| Title |
|
|
|
Rapid identification of thermotolerant Campylobacter jejuni, Campylobacter coli, Campylobacter lari, and Campylobacter upsaliensis from various geographic locations by a GTPase-based PCR-reverse hybridization assay
| |
| Author |
|
|
|
| |
| Abstract |
|
|
|
| Recently, a gene from Campylobacter jejuni encoding a putative GTPase was identified. Based on two semiconserved GTP-binding sites encoded within this gene, PCR primers were selected that allow amplification of a 153-bp fragment from C. jejuni, C. coli, C. lari, and C. upsaliensis. Sequence analysis of these PCR products revealed consistent interspecies variation, which allowed the definition of species-specific probes for each of the four thermotolerant Campylobacter species. Multiple probes were used to develop a line probe assay (LiPA) that permits analysis of PCR products by a single reverse hybridization step. A total of 320 reference strains and clinical isolates from various geographic origins were tested by the GTP-based PCR-LiPA. The PCR-LiPA is highly specific in comparison with conventional identification methods, including biochemical and whole-cell protein analyses. In conclusion, a simple method has been developed for rapid and highly specific identification of thermotolerant Campylobacter species. | | |
| Language |
|
|
|
English
| |
| Source (journal) |
|
|
|
Journal of clinical microbiology. - Washington, D.C. | |
| Publication |
|
|
|
Washington, D.C. : 1999
| |
| ISSN |
|
|
|
0095-1137
| |
| Volume/pages |
|
|
|
37:6(1999), p. 1790-1796
| |
| ISI |
|
|
|
000080344900022
| |
| Full text (publisher's version - intranet only) |
|
|
| | |
|