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Title
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Rapid identification of thermotolerant Campylobacter jejuni, Campylobacter coli, Campylobacter lari, and Campylobacter upsaliensis from various geographic locations by a GTPase-based PCR-reverse hybridization assay
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Author
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Abstract
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| Recently, a gene from Campylobacter jejuni encoding a putative GTPase was identified. Based on two semiconserved GTP-binding sites encoded within this gene, PCR primers were selected that allow amplification of a 153-bp fragment from C. jejuni, C. coli, C. lari, and C. upsaliensis. Sequence analysis of these PCR products revealed consistent interspecies variation, which allowed the definition of species-specific probes for each of the four thermotolerant Campylobacter species. Multiple probes were used to develop a line probe assay (LiPA) that permits analysis of PCR products by a single reverse hybridization step. A total of 320 reference strains and clinical isolates from various geographic origins were tested by the GTP-based PCR-LiPA. The PCR-LiPA is highly specific in comparison with conventional identification methods, including biochemical and whole-cell protein analyses. In conclusion, a simple method has been developed for rapid and highly specific identification of thermotolerant Campylobacter species. |
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Language
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English
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Source (journal)
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Journal of clinical microbiology. - Washington, D.C.
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Publication
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Washington, D.C.
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1999
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ISSN
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0095-1137
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DOI
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10.1128/JCM.37.6.1790-1796.1999
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Volume/pages
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37
:6
(1999)
, p. 1790-1796
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ISI
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000080344900022
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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