Title
Carrier mediated uptake of dehydroascorbate into higher plant plasma membrane vesicles shows trans-stimulation Carrier mediated uptake of dehydroascorbate into higher plant plasma membrane vesicles shows trans-stimulation
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Amsterdam ,
Subject
Physics
Chemistry
Biology
Human medicine
Source (journal)
FEBS letters: for the rapid publication of short reports in biochemistry, biophysics and molecular biology / Federation of European Biochemical Societies. - Amsterdam
Volume/pages
421(1998) :1 , p. 41-44
ISSN
0014-5793
ISI
000071696200010
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The activity of the ascorbate (Asc) carrier of purified Phaseolus plasma membranes is demonstrated to be highly stimulated when membrane vesicles are preloaded with Asc. Asc transport is inhibited by DTT but is not affected by glutathione or ferricyanide, indicating that dehydroascorbate (DHA) is the preferred species for uptake, Asc transport in the loaded vesicles showed saturable kinetics with an apparent affinity constant of 24 mu M and maximal uptake rate of 94 pmol/mg/min. Addition of DHA stimulated the efflux of Asc molecules from the loaded vesicles. Together these results suggest the presence of an Asci DHA exchange mechanism in higher plant: plasma membranes. (C) 1998 Federation of European Biochemical Societies.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/36dac8/4985.pdf
E-info
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