Publication
Title
Carrier mediated uptake of dehydroascorbate into higher plant plasma membrane vesicles shows trans-stimulation
Author
Abstract
The activity of the ascorbate (Asc) carrier of purified Phaseolus plasma membranes is demonstrated to be highly stimulated when membrane vesicles are preloaded with Asc. Asc transport is inhibited by DTT but is not affected by glutathione or ferricyanide, indicating that dehydroascorbate (DHA) is the preferred species for uptake, Asc transport in the loaded vesicles showed saturable kinetics with an apparent affinity constant of 24 mu M and maximal uptake rate of 94 pmol/mg/min. Addition of DHA stimulated the efflux of Asc molecules from the loaded vesicles. Together these results suggest the presence of an Asci DHA exchange mechanism in higher plant: plasma membranes. (C) 1998 Federation of European Biochemical Societies.
Language
English
Source (journal)
FEBS letters: for the rapid publication of short reports in biochemistry, biophysics and molecular biology / Federation of European Biochemical Societies. - Amsterdam
Publication
Amsterdam : 1998
ISSN
0014-5793
Volume/pages
421:1(1998), p. 41-44
ISI
000071696200010
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 03.01.2013
Last edited 10.06.2017
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