Title
Simultaneous separation and determination of polychlorinated biphenyl congeners and other chlorinated hydrocarbon residues in human matrices using gel permeation or adsorption chromatographic clean-up and GC-MS quantification Simultaneous separation and determination of polychlorinated biphenyl congeners and other chlorinated hydrocarbon residues in human matrices using gel permeation or adsorption chromatographic clean-up and GC-MS quantification
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Publication type
article
Publication
New York, N.Y. ,
Subject
Chemistry
Biology
Source (journal)
International journal of environmental analytical chemistry. - New York, N.Y.
Volume/pages
71(1998) :2 , p. 105-118
ISSN
0306-7319
ISI
000078611900001
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Two methods are described for the analysis of organochlorine compounds (OCs) in human matrices (adipose tissue and serum). These comprise the extraction, clean-up and quantification by gas chromatography-mass spectrometry in Selected Ion Monitoring-mode. The procedures involve liquid-liquid extraction for serum and column percolation for adipose tissue. The serum clean-up involves adsorption chromatography through deactivated silica gel. The solvent system, consisting of 30 mL n-hexane, followed by 10 mL n-hexane:dichloromethane (80:20, v/v) and 10 mL n-hexane:dichloromethane (40:60, v/v), gives acceptable recoveries, but is not suitable for the extraction of dieldrin and lindane (gamma-HCH). An optimisation of gel permeation chromatography (GPC) is presented for separation of OCs from extractable adipose tissue lipids. The high efficiency of the GPC provides very clean extracts for GC-MS analysis and yields high recoveries for the individual compounds studied (mean recovery 89 % for pesticides and polychlorinated biphenyls).
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