|
|
|
Title
|
|
|
|
The predominance of Human Immunodeficiency Virus type I (HIV-I) circulating recombinant form 02 (CRF02_AG) in West Central Africa may be related to its replicative fitness
| |
|
Author
|
|
|
|
| |
|
Abstract
|
|
|
|
| Background: CRF02_AG is the predominant HIV strain circulating in West and West Central Africa. The aim of this study was to test whether this predominance is associated with a higher in vitro replicative fitness relative to parental subtype A and G viruses. Primary HIV-1 isolates (10 CRF02_AG, 5 subtype A and 5 subtype G) were obtained from a well-described Cameroonian cohort. Growth competition experiments were carried out at equal multiplicity of infection in activated T cells and monocyte-derived dendritic cells (MO-DC) in parallel. Results: Dual infection/competition experiments in activated T cells clearly indicated that CRF02_AG isolates had a significant replication advantage over the subtype A and subtype G viruses. The higher fitness of CRF02_AG was evident for isolates from patients with CD4+T cell counts > 200 cells/mu L (non-AIDS) or CD4+T cell counts < 200 cells/mu L ( AIDS), and was independent of the co-receptor tropism. In MO-DC cultures, CRF02_AG isolates showed a slightly but not significantly higher replication advantage compared to subtype A or G isolates. Conclusion: We observed a higher ex vivo replicative fitness of CRF02_AG isolates compared to subtype A and G viruses from the same geographic region and showed that this was independent of the co-receptor tropism and irrespective of high or low CD4+T cell count. This advantage in replicative fitness may contribute to the dominant spread of CRF02_AG over A and G subtypes in West and West Central Africa. |
| |
|
Language
|
|
|
|
English
| |
|
Source (journal)
|
|
|
|
Retrovirology. - London
| |
|
Publication
|
|
|
|
London
:
2006
| |
|
ISSN
|
|
|
|
1742-4690
| |
|
Volume/pages
|
|
|
|
3
(2006)
, p. 1-11
| |
|
Article Reference
|
|
|
|
40
| |
|
ISI
|
|
|
|
000239812100002
| |
|
Medium
|
|
|
|
E-only publicatie
| |
|
Full text (Publisher's DOI)
|
|
|
|
| |
|
Full text (open access)
|
|
|
|
| |
|
|
Faculty/Department
|
|
|
|
| |
|
Research group
|
|
|
| | |
|
Subject
|
|
|
|
| |
|
Affiliation
|
|
|
|
Publications with a UAntwerp address
| |
|
|
|
|
Identification
|
|
| |
|
|
|
Creation
|
|
| |
03.01.2013
|
|
|
Last edited
|
|
| |
09.12.2021
|
|
|
To cite this reference
|
|
|
|
| |
|
| |