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Title
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Bcl-2 protects against FCCP-induced apoptosis and mitochondrial membrane potential depolarization in PC12 cells
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Author
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Abstract
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| This report addresses the relation between Bcl-2 and mitochondrial membrane potential (Delta Psi(m)) in apoptotic cell death. Rat pheochromocytoma (PC12) cells are differentiated into neuron-like cells with nerve growth factor (NGF). It is known that Bcl-2 can attenuate apoptosis induced by deprivation of neurotrophic factor. The protective effect of Bcl-2 has been correlated with preservation of Delta Psi(m). Protonophores, such as carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP), collapse the proton gradient across the mitochondrial inner membrane, resulting in a complete abolition of the mitochondrial membrane potential. Based on the analysis of morphology, of phosphatidylserine exposure and of nuclear fragmentation we conclude that FCCP induces apoptosis in PC12 cells, which can be prevented by overexpression of Bcl-2. To determine whether the cytoprotective effect of Bcl-2 is due to stabilization of Delta Psi(m), we investigated the effect of Bcl-2 on changes in Delta Psi(m), induced by FCCP in PC12 cells. We showed that treatment with FCCP induced a reduction in Delta Psi(m), as assessed with the lipophilic cationic membrane potential-sensitive dye JC-1, and that Bcl-2 protects against FCCP-induced changes in NGF differentiated PC12 cells. Our data indicate that Bcl-2 protects against FCCP-induced cell death by stabilizing Delta Psi(m). (C) 1999 Elsevier Science B.V. All rights reserved. |
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Language
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English
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Source (journal)
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Biochimica et biophysica acta : G : general subjects. - Amsterdam, 1964, currens
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Publication
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Amsterdam
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Elsevier
,
1999
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ISSN
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0304-4165
[print]
1872-8006
[online]
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DOI
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10.1016/S0304-4165(99)00073-2
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Volume/pages
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1428
:2-3
(1999)
, p. 357-371
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ISI
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000082142200026
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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