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Title
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Structural basis of eye lens transparency : light scattering by concentrated solutions of bovine alpha-crystallin proteins
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Author
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Abstract
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| Short range order of the crystallins does account for the transparency of the eye lens, To explain the solution structure of this highly concentrated protein solution on a quantitative basis, the hydrodynamic structure and the interparticle interactions of the proteins have to be known. For that purpose, the light scattering of concentrated solutions of alpha-crystallin has been studied. Starting from the detailed knowledge of the solution parameters of alpha-crystallin in diluted solutions, the structure of concentrated solutions up to 360 mg/ml has been studied using light scattering. Our results indicate that subtle changes in the macromolecular structure such as optical anisotropy or structural asymmetry for part of the alpha-crystallins, which results in solute light-scattering heterogeneity, can dramatically increase the light scattering by the alpha-crystallins and cause solution opacity. |
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Language
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English
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Source (journal)
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Biophysical journal. - New York, N.Y.
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Publication
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New York, N.Y.
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1996
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ISSN
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0006-3495
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Volume/pages
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71
:5
(1996)
, p. 2815-2822
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ISI
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A1996VQ52000052
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Full text (Publisher's DOI)
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Full text (open access)
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