Publication
Title
Polymerase chain reaction-mediated dna-fingerprinting for epidemiologic studies on campylobacter spp
Author
Abstract
The applicability of polymerase chain reaction (PCR)-mediated DNA typing, with primers complementary to dispersed repetitive DNA sequences and arbitrarily chosen DNA motifs, to study the epidemiology of campylobacter infection was evaluated. With a single PCR reaction and simple gel electrophoresis, strain-specific DNA banding patterns were observed for Campylobacter jejuni and C. upsaliensis. DNA from multiple strains isolated during an outbreak of C. jejuni meningitis generated identical banding patterns and could be distinguished from randomly isolated strains. Strains from a community outbreak of C. upsaliensis, that were all identical by conventional typing methods, could be divided into two genetically different groups. This report illustrates that PCR fingerprinting can be successfully applied in epidemiological investigations of campylobacter infections.
Language
English
Source (journal)
Journal of medical microbiology. - Edinburgh, 1968, currens
Publication
Edinburgh : 1994
ISSN
0022-2615
DOI
10.1099/00222615-40-2-141
Volume/pages
40 :2 (1994) , p. 141-147
ISI
A1994MX15400013
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 03.01.2013
Last edited 04.03.2024
To cite this reference