Title
Polymerase chain reaction-mediated dna-fingerprinting for epidemiologic studies on campylobacter spp Polymerase chain reaction-mediated dna-fingerprinting for epidemiologic studies on campylobacter spp
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Edinburgh ,
Subject
Biology
Source (journal)
Journal of medical microbiology. - Edinburgh, 1968, currens
Volume/pages
40(1994) :2 , p. 141-147
ISSN
0022-2615
ISI
A1994MX15400013
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The applicability of polymerase chain reaction (PCR)-mediated DNA typing, with primers complementary to dispersed repetitive DNA sequences and arbitrarily chosen DNA motifs, to study the epidemiology of campylobacter infection was evaluated. With a single PCR reaction and simple gel electrophoresis, strain-specific DNA banding patterns were observed for Campylobacter jejuni and C. upsaliensis. DNA from multiple strains isolated during an outbreak of C. jejuni meningitis generated identical banding patterns and could be distinguished from randomly isolated strains. Strains from a community outbreak of C. upsaliensis, that were all identical by conventional typing methods, could be divided into two genetically different groups. This report illustrates that PCR fingerprinting can be successfully applied in epidemiological investigations of campylobacter infections.
E-info
https://repository.uantwerpen.be/docman/iruaauth/c64f99/83c4113.pdf
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1994MX15400013&DestLinkType=RelatedRecords&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1994MX15400013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1994MX15400013&DestLinkType=CitingArticles&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848
Handle