Title
Contribution of <tex>$\alpha$</tex>-adrenoceptor stimulation by phenylephrine to basal nitric oxide production in the isolated mouse aorta Contribution of <tex>$\alpha$</tex>-adrenoceptor stimulation by phenylephrine to basal nitric oxide production in the isolated mouse aorta
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Faculty of Medicine and Health Sciences
Publication type
article
Publication
New York ,
Subject
Pharmacology. Therapy
Human medicine
Source (journal)
Journal of cardiovascular pharmacology. - New York
Volume/pages
61(2013) :4 , p. 318-323
ISSN
0160-2446
ISI
000317480300008
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
In the mouse aorta, contractions evoked by the [alpha]1-adrenoceptor agonist phenylephrine are strongly suppressed by the continuous production of nitric oxide (NO). We investigated whether phenylephrine itself stimulated NO production by activating endothelial [alpha]2-adrenoceptors. On a prostaglandin F2[alpha] contraction, the [alpha]2-adrenoceptor agonist 5-bromo-N-(4,5-dihydro-1H-imidazol-2-yl)-6-quinoxalinamine (UK14304) induced 29.3+/-7.4% relaxation which was inhibited by 0.1 [micro]M 2-[(4,5-Dihydro-1H-imidazol-2-yl)methyl]-2,3-dihydro-1-methyl-1H-isoindole (BRL44408) with a pKB' corresponding to [alpha]2-antagonism. In the presence of NO synthase blockers, UK14304 elicited small contractions above 1 [micro]M which were inhibited by 0.1 [micro]M prazosin, but not influenced by 0.1 [micro]M rauwolscine. At 3 [micro]M or higher concentrations, phenylephrine caused only modest relaxation (up to 7.4+/-2.3%) of segments constricted with prostaglandin F2[alpha] in the presence of prazosin, which was abolished with 0.1 [micro]M BRL44408. Furthermore, BRL44408 did not increase contractions induced with 1 [micro]M phenylephrine. These results confirm that [alpha]1- but not [alpha]2-adrenoceptors are expressed on aortic smooth muscle cells, while endothelial cells only express [alpha]2-adrenoceptors. Moreover, phenylephrine exerted a very modest relaxing effect through non-specific stimulation of [alpha]2-adrenoceptors, but only at concentrations higher than 1 [micro]M. It is concluded that the high basal output of NO in the isolated mouse aorta is not due to stimulation of [alpha]-adrenoceptors.
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