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Title
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Thioquinolobactin, a **Pseudomonas** siderophore with antifungal and anti-**Pythium** activity
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Author
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Abstract
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| Under conditions of iron limitation Pseudomonas fluorescens ATCC 17400 produces two siderophores, pyoverdine, and a second siderophore quinolobactin, which itself results from the hydrolysis of the unstable molecule 8-hydroxy-4-methoxy-2-quinoline thiocarboxylic acid (thioquinolobactin). Pseudomonas fluorescens ATCC 17400 also displays a strong in vitro antagonism against the Oomycete Pythium, which is repressed by iron, suggesting the involvement of a siderophore(s). While a pyoverdine-negative mutant retains most of its antagonism, a thioquinolobactin-negative mutant only slowed-down Pythium growth, and a double pyoverdine-, thioquinolobactin-negative mutant, which does not produce any siderophore, totally lost its antagonism against Pythium. The siderophore thioquinolobactin could be purified and identified from spent medium and showed anti-Pythium activity, but it was quickly hydrolysed to quinolobactin, which we showed has no antimicrobial activity. Analysis of antagonism-affected transposon mutants revealed that genes involved in haem biosynthesis and sulfur assimilation are important for the production of thioquinolobactin and the expression of antagonism. |
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Language
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English
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Source (journal)
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Environmental microbiology. - Oxford
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Publication
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Oxford
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2007
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ISSN
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1462-2912
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DOI
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10.1111/J.1462-2920.2006.01154.X
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Volume/pages
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9
:2
(2007)
, p. 425-434
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ISI
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000243376800014
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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