Publication
Title
Accumulation capacity of primary cultures of adipocytes for PCB-126 : influence of cell differentiation stage and triglyceride levels
Author
Abstract
Polychlorinated biphenyls (PCBs) are lipophilic persistent organic chemicals that accumulate at high concentrations in the adipose tissue. Recent studies correlate the presence of such contaminants in fat cells to possible alterations in the regulation of energy homeostasis in adipocytes. As the adipose tissue is composed of adipocytes at several stages of differentiation, it is possible that PCBs already accumulate in cells at an early stage, and thereby impair their development. The exact driving force enabling the massive accumulation of PCBs in fat cells remains unclear. The present study investigated the time-course incorporation of H-3-PCB-126 in primary cultures of rat adipocytes at both early and late differentiation stages and showed that the accumulation of this congener was already significant at an early stage of differentiation. In addition, triglyceride levels in cells were an important parameter governing H-3-PCB-126's entry. The extent of adipocyte ability to store this pollutant in vitro was also evaluated and revealed that fat cells were able to accumulate H-3-PCB-126 at extremely high concentrations. A linear relationship was observed between the amount of H-3-PCB-126 added to the medium and the one accumulated in the cells, which favors a passive diffusion mechanism for the entry of this pollutant into fat cells. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
Language
English
Source (journal)
Toxicology letters. - Amsterdam
Publication
Amsterdam : 2012
ISSN
0378-4274
Volume/pages
214:3(2012), p. 243-250
ISI
000310534100002
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 04.02.2013
Last edited 19.06.2017
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