Title
Testing chemical agents with the cytokinesis-block micronucleus cytome assay
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Praha ,
Subject
Chemistry
Biology
Human medicine
Source (journal)
Folia biologica. - Praha
Volume/pages
58(2012) :5 , p. 215-220
ISSN
0015-5500
ISI
000312058500006
Carrier
E
Target language
English (eng)
Affiliation
University of Antwerp
Abstract
In order to evaluate the applicability of the cytokinesis-block micronucleus cytome assay in routine mutagenicity testing we investigated with this method different chemicals having different mechanisms of action: non-mutagens, direct-acting base-altering mutagens, direct-acting cross-linking mutagens, clastogens including a radiomimetic chemical, indirect-acting spindle poisons and indirect-acting enzyme inhibitors. We looked at the presence of micronuclei as biomarkers for either the lost; of chromosome fragments (clastogen) or the loss of a whole chromosome (aneugen), nucleoplasmic bridges as biomarkers for complex rearrangements (e.g., dicentric chromosomes) and nuclear buds as biomarkers for gene amplification. The cytome assay proved to be a suitable tool to investigate genetic effects of environmental agents and to provide insight into their working mechanisms as all chemicals tested showed the expected response.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/99dbae/3721.pdf
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