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Title
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Testing chemical agents with the cytokinesis-block micronucleus cytome assay
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Author
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Abstract
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| In order to evaluate the applicability of the cytokinesis-block micronucleus cytome assay in routine mutagenicity testing we investigated with this method different chemicals having different mechanisms of action: non-mutagens, direct-acting base-altering mutagens, direct-acting cross-linking mutagens, clastogens including a radiomimetic chemical, indirect-acting spindle poisons and indirect-acting enzyme inhibitors. We looked at the presence of micronuclei as biomarkers for either the lost; of chromosome fragments (clastogen) or the loss of a whole chromosome (aneugen), nucleoplasmic bridges as biomarkers for complex rearrangements (e.g., dicentric chromosomes) and nuclear buds as biomarkers for gene amplification. The cytome assay proved to be a suitable tool to investigate genetic effects of environmental agents and to provide insight into their working mechanisms as all chemicals tested showed the expected response. |
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Language
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English
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Source (journal)
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Folia biologica. - Praha
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Publication
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Praha
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2012
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ISSN
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0015-5500
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Volume/pages
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58
:5
(2012)
, p. 215-220
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ISI
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000312058500006
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Full text (open access)
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