Title
Isolation and characterisation of a banana CYCD2;1 gene and its over-expression enhances root growth Isolation and characterisation of a banana CYCD2;1 gene and its over-expression enhances root growth
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Subject
Biology
Source (journal)
African journal of biotechnology. - -
Volume/pages
11(2012) :45 , p. 10328-10339
ISSN
1684-5315
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Stimulation of the cell cycle by over-expression of Arabidopsis CyclinD2;1 enhanced growth in tobacco and rice, but not Arabidopsis. To broaden the range of species and to improve an important crop species for developing countries, a CyclinD2;1 homologue was isolated from an East African highland banana cultivar Nakasabira (Musa spp., AAA group) and designated Musac;CYCD2;1 (Genebank accession no HQ839770). Musac;CYCD2;1 amino-acid sequence exhibited 40, 54, 57 and 57% identity with CYCD2;1 sequences of Arabidopsis, rice, maize and wheat, respectively. Over-expression of Musac;CYCD2;1 driven by the CaMV 35S promoter in cultivar Sukali ndizi (AAB) resulted in transformed plants with no significant increase in total banana CYCD2;1 transcripts in the shoot, but up to 66-fold increase of total banana CYCD2;1 transcripts in roots. Correspondingly, transformed plants had no change in above-ground growth but produced longer main roots and more lateral roots under in vitro conditions. A deeper root system was observed in one transgenic line relative to non-transformed plants when grown in soil. Results demonstrate that a banana homologue of Arabidopsis CYCD2;1 can also enhance growth in a homologous background and the potential of enhancing banana root growth.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/4842b3/6b6b7c84.pdf
Handle