Title
Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Baltimore, Md ,
Subject
Biology
Source (journal)
Journal of biological chemistry. - Baltimore, Md
Volume/pages
282(2007) , p. 25588-25596
ISSN
0021-9258
ISI
000249014100045
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
The EL2 gene of rice (Oryza sativa), previously classified as early response gene against the potent biotic elicitor N-acetylchitoheptaose and encoding a short polypeptide with unknown function, was identified as a novel cell cycle regulatory gene related to the recently reported SIAMESE (SIM) gene of Arabidopsis thaliana. Iterative two-hybrid screens, in vitro pull-down assays, and fluorescence resonance energy transfer analyses showed that Orysa; EL2 binds the cyclin-dependent kinase (CDK) CDKA1;1 and D-type cyclins. No interaction was observed with the plant-specific B-type CDKs. The amino acid motif ELERFL was identified to be essential for cyclin, but not for CDK binding. Orysa;EL2 impaired the ability of Orysa; CYCD5;3 to complement a budding yeast (Saccharomyces cerevisiae) triple CLN mutant, whereas recombinant protein inhibited CDK activity in vitro. Moreover, Orysa;EL2 was able to rescue the multicellular trichome phenotype of sim mutants of Arabidopsis, unequivocally demonstrating that Orysa;EL2 operates as a cell cycle inhibitor. Orysa;EL2 mRNA levels were induced by cold, drought, and propionic acid. Our data suggest that Orysa;EL2 encodes a new type of plant CDK inhibitor that links cell cycle progression with biotic and abiotic stress responses.
E-info
https://repository.uantwerpen.be/docman/iruaauth/4657d0/85e58d522db.pdf
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