Title
PDF receptor signaling in **Caenorhabditis elegans** modulates locomotion and egg-laying
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Amsterdam ,
Subject
Biology
Human medicine
Source (journal)
Molecular and cellular endocrinology. - Amsterdam
Volume/pages
361(2012) :1-2 , p. 232-240
ISSN
0303-7207
ISI
000307692200024
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
In Caenorhabdiris elegans, pdfr-1 encodes three receptors of the secretin receptor family. These G protein-coupled receptors are activated by three neuropeptides, pigment dispersing factors 1a, 1b and 2, which are encoded by pdf-1 and pdf-2. We isolated a PDF receptor loss-of-function allele (lst34) by means of a mutagenesis screen and show that the PDF signaling system is involved in locomotion and egg-laying. We demonstrate that the pdfr-1 mutant phenocopies the defective locomotor behavior of the pdf-1 mutant and that pdf-1 and pdf-2 behave antagonistically. All three PDF receptor splice variants are involved in the regulation of locomotor behavior. Cell specific rescue experiments show that this pdf mediated behavior is regulated by neurons rather than body wall muscles. We also show that egg-laying patterns of pdf-1 and pdf-2 mutants are affected, but not those of pdfr-1 mutants, pointing to a novel role for the PDF-system in the regulation of egg-laying. (c) 2012 Elsevier Ireland Ltd. All rights reserved.
E-info
https://repository.uantwerpen.be/docman/iruaauth/664824/22a2984.pdf
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