Title
UNC-108/RAB-2 and its effector RIC-19 are involved in dense core vesicle maturation in **Caenorhabditis elegans** UNC-108/RAB-2 and its effector RIC-19 are involved in dense core vesicle maturation in **Caenorhabditis elegans**
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
New York ,
Subject
Biology
Human medicine
Source (journal)
The journal of cell biology / American Society for Cell Biology. - New York
Volume/pages
186(2009) :6 , p. 897-914
ISSN
0021-9525
ISI
000269960800013
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Small guanosine triphosphatases of the Rab family regulate intracellular vesicular trafficking. Rab2 is highly expressed in the nervous system, yet its function in neurons is unknown. In Caenorhabditis elegans, unc-108/rab-2 mutants have been isolated based on their locomotory defects. We show that the locomotion defects of rab-2 mutants are not caused by defects in synaptic vesicle release but by defects in dense core vesicle (DCV) signaling. DCVs in rab-2 mutants are often enlarged and heterogeneous in size; however, their number and distribution are not affected. This implicates Rab2 in the biogenesis of DCVs at the Golgi complex. We demonstrate that Rab2 is required to prevent DCV cargo from inappropriately entering late endosomal compartments during DCV maturation. Finally, we show that RIC-19, the C. elegans orthologue of the human diabetes autoantigen ICA69, is also involved in DCV maturation and is recruited to Golgi membranes by activated RAB-2. Thus, we propose that RAB-2 and its effector RIC-19 are required for neuronal DCV maturation.
E-info
https://repository.uantwerpen.be/docman/iruaauth/de481a/9442992.pdf
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