|
Title
|
|
|
|
Altered neuropeptide profile of **Caenorhabditis elegans** lacking the chaperone protein 7B2 as analyzed by mass spectrometry
|
|
|
Author
|
|
|
|
|
|
|
Abstract
|
|
|
|
| Cellular synthesis of naturally occurring, bioactive peptides requires the proprotein convertase PC2/EGL-3 for cleavage from the larger peptide precursors. A neuroendocrine chaperone 7132 is needed for the proteolytical activation of proPC2, as extensively studied in mouse models. To determine the role of its orthologue in Caenorhabditis elegans, we analyzed wild-type and 7132-null strains by HPLC and matrix-assisted laser desorption ionization time-of-flight mass spectrometry, which allowed the identification of a novel neuropeptide gene, flp-33. The presence and/or absence of some neuropeptides in 7132-null animals strongly differs form the peptide profile in wild-type, suggesting a specific and determined action of 7132 in C. elegans. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
|
|
|
Language
|
|
|
|
English
|
|
|
Source (journal)
|
|
|
|
FEBS letters: for the rapid publication of short reports in biochemistry, biophysics and molecular biology / Federation of European Biochemical Societies. - Amsterdam
|
|
|
Publication
|
|
|
|
Amsterdam
:
2007
|
|
|
ISSN
|
|
|
|
0014-5793
|
|
|
Volume/pages
|
|
|
|
581
:22
(2007)
, p. 4288-4292
|
|
|
ISI
|
|
|
|
000249476900027
|
|
|
Full text (Publisher's DOI)
|
|
|
|
|
|
|
Full text (publisher's version - intranet only)
|
|
|
|
|
|