Title
Altered neuropeptide profile of **Caenorhabditis elegans** lacking the chaperone protein 7B2 as analyzed by mass spectrometry Altered neuropeptide profile of **Caenorhabditis elegans** lacking the chaperone protein 7B2 as analyzed by mass spectrometry
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Amsterdam ,
Subject
Physics
Chemistry
Biology
Human medicine
Source (journal)
FEBS letters: for the rapid publication of short reports in biochemistry, biophysics and molecular biology / Federation of European Biochemical Societies. - Amsterdam
Volume/pages
581(2007) :22 , p. 4288-4292
ISSN
0014-5793
ISI
000249476900027
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Cellular synthesis of naturally occurring, bioactive peptides requires the proprotein convertase PC2/EGL-3 for cleavage from the larger peptide precursors. A neuroendocrine chaperone 7132 is needed for the proteolytical activation of proPC2, as extensively studied in mouse models. To determine the role of its orthologue in Caenorhabditis elegans, we analyzed wild-type and 7132-null strains by HPLC and matrix-assisted laser desorption ionization time-of-flight mass spectrometry, which allowed the identification of a novel neuropeptide gene, flp-33. The presence and/or absence of some neuropeptides in 7132-null animals strongly differs form the peptide profile in wild-type, suggesting a specific and determined action of 7132 in C. elegans. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
E-info
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