Publication
Title
Identification and relative quantification of neuropeptides from the endocrine tissues
Author
Abstract
Endocrine tissues like the pituitary, hypothalamus and islets of Langerhans are rich in bioactive peptides. These are used for intercellular signalling and are involved in regulation of almost all physiological processes. Peptidomics is the comprehensive analysis of peptides in tissues, fluids and cells. Peptidomics applied to (neuro-)endocrine tissues aims therefore to identify as many bioactive peptides as possible. Peptidomics of (neuro-)endocrine tissues requires an integrated approach that consists of careful sample handling, peptide separation techniques, mass spectrometry and bioinformatics. Here we describe the methods for isolation and dissection of endocrine tissues, the extraction of bioactive peptides and further sample handling and identification of peptides by mass spectrometry and hyphenated techniques. We also present a straightforward method for the comparison of relative levels of bioactive peptides in these endocrine tissues under varying physiological conditions. The latter helps to elucidate functions of the bioactive peptides.
Language
English
Source (journal)
Methods in molecular biology. - Totowa, N.J., 1984, currens
Publication
Totowa, N.J. : 2010
ISSN
1064-3745 [print]
1940-6029 [online]
Volume/pages
615(2010), p. 191-206
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Publication type
Subject
External links
Record
Identification
Creation 14.03.2013
Last edited 14.03.2016