Publication
Title
Colonoscopy and are valid techniques to monitor inflammation in the adoptive transfer colitis model in mice
Author
Abstract
Background: Preclinical in vivo research on inflammatory bowel diseases requires proper animal models and techniques allowing longitudinal monitoring of colonic inflammation without the need to kill animals. We evaluated colonoscopy and [mu]-positron emission tomography/computed tomography ([mu]PET/CT) as monitoring tools in a model for chronic colitis in mice. Methods: Colitis was induced by adoptive transfer of CD4+CD25-CD62L+ T cells in immunocompromised severe combined immunodeficient mice. Three study protocols were designed. In study 1, colonoscopy and [micro]PET/CT were performed once, 4 weeks after transfer. In study 2 and study 3, colitis was sequentially followed up through colonoscopy (study 2) or colonoscopy plus [micro]PET/CT (study 3). Each study included postmortem evaluation of colonic inflammation (macroscopy, microscopy, and myeloperoxidase activity). Results: In study 1, both colonoscopy and [micro]PET/CT detected colitis 4 weeks after transfer. Study 2 showed a gradual increase in colonoscopic score from week 2 (1.4 +/- 0.6) to week 8 (6.0 +/- 1.1). In study 3, colitis was detected 2 weeks after transfer by [micro]PET/CT (2.0 +/- 0.4) but not by colonoscopy, whereas both techniques detected inflammation 4 and 6 weeks after transfer. Colonoscopy correlated with [micro]PET/CT (r = 0.812, 0.884, and 0.781, respectively) and with postmortem analyses in all 3 studies. Conclusions: Adoptive transfer of CD4+CD25-CD62L+ T cells in severe combined immunodeficient mice results in a moderate chronic colitis. Evolution of colitis could be monitored over time by both colonoscopy and [micro]PET/CT. [micro]PET/CT seems to detect inflammation at an earlier time point than colonoscopy. Both techniques represent reliable and safe methods without the need to kill animals.
Language
English
Source (journal)
Inflammatory bowel diseases. - New York, N.Y.
Publication
New York, N.Y. : 2013
ISSN
1078-0998
Volume/pages
19:5(2013), p. 967-976
ISI
000336084200021
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 02.04.2013
Last edited 16.11.2017
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