Title
Reductive nitrosylation of **Methanosarcina acetivorans** protoglobin : a comparative study Reductive nitrosylation of **Methanosarcina acetivorans** protoglobin : a comparative study
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
New York, N.Y. ,
Subject
Physics
Chemistry
Biology
Human medicine
Source (journal)
Biochemical and biophysical research communications. - New York, N.Y.
Volume/pages
430(2013) :4 , p. 1301-1305
ISSN
0006-291X
ISI
000315075200020
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Methanosarcina acetivorans is a strictly anaerobic non-motile methane-producing Archaea expressing protoglobin (Pgb) which might either facilitate O2 detoxification or act as a CO sensor/supplier in methanogenesis. Unusually, M. acetivorans Pgb (MaPgb) binds preferentially O2 rather than CO and displays anticooperativity in ligand binding. Here, kinetics and/or thermodynamics of ferric and ferrous MaPgb (MaPgb(III) and MaPgb(II), respectively) nitrosylation are reported. Data were obtained between pH 7.2 and 9.5, at 22.0 °C. Addition of NO to MaPgb(III) leads to the transient formation of MaPgb(III)NO in equilibrium with MaPgb(II)NO+. In turn, MaPgb(II)NO+ is converted to MaPgb(II) by OH−-based catalysis. Then, MaPgb(II) binds NO very rapidly leading to MaPgb(II)NO. The rate-limiting step for reductive nitrosylation of MaPgb(III) is represented by the OH−-mediated reduction of MaPgb(II)NO+ to MaPgb(II). Present results highlight the potential role of MaPgb in scavenging of reactive nitrogen and oxygen species.
E-info
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