Title
Effects of vitamin C and <tex>$\beta$</tex>-carotene pre-treatment on liver damage caused by short-term <tex>$CCl_{4}$</tex> administration in rats Effects of vitamin C and <tex>$\beta$</tex>-carotene pre-treatment on liver damage caused by short-term <tex>$CCl_{4}$</tex> administration in rats
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Subject
Human medicine
Source (journal)
International journal of biological and chemical sciences
Volume/pages
5(2011) :2 , p. 824-834
ISSN
1991-8631
Carrier
E
Target language
English (eng)
Affiliation
University of Antwerp
Abstract
The protective role of antioxidant vitamins on drug/alcohol induced liver injury is increasingly being demonstrated in animal models, thus lending substantive support for the use of vitamin supplements in humans. The effects of vitamin C and β-carotene in modulating liver damage caused by CCl4 (carbon tetrachloride) poisoning in rats were recently examined. Thirty two male albino rats were allocated to 4 groups: Group 1 received intramuscular (IM) injections of saline daily; Group 2 was injected intraperitoneally (IP) with CCl4 every third day for 3 weeks; Groups 3 and 4 were treated with daily IM injections of vitamin C and β-carotene, respectively, starting 5 days before co-administration with CCl4. Compared to group 2, alanine aminotransferase was lower in groups 3 and 4 without, however, reaching statistical significance (respectively, p=0.066 and 0.139), whereas gamma glutamyltransferase level and total bilirubin concentration in the same groups were significantly lower (p<0.001). Vitamin C pre-treatment in group 3 caused significantly lower histology scores (p<0.05) for necrosis, steatosis and fibrosis as compared to untreated group. β-Carotene pretreatment exhibited no significant effect. These results suggest that prophylactic doses of vitamin C are largely protective against short term chemical liver injury in rats, but in the same setting, we found β-carotene less protective.
E-info
https://repository.uantwerpen.be/docman/iruaauth/1f4917/8a0ccfab217.pdf
Handle