Title
MicroCT versus sTSLIM 3D imaging of the mouse cochleaMicroCT versus sTSLIM 3D imaging of the mouse cochlea
Author
Faculty/Department
Faculty of Sciences. Physics
Research group
Biophysics and Biomedical Physics
Publication type
article
Publication
New York, N.Y.,
Subject
Physics
Biology
Human medicine
Source (journal)
The journal of histochemistry and cytochemistry. - New York, N.Y.
Volume/pages
61(2013):5, p. 382-395
ISSN
0022-1554
ISI
000319684400006
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
We made a qualitative and quantitative comparison between a state-of-the-art implementation of micro-Computed Tomography (microCT) and the scanning Thin-Sheet Laser Imaging Microscopy (sTSLIM) method, applied to mouse cochleae. Both imaging methods are non-destructive and perform optical sectioning, respectively, with X-rays and laser light. MicroCT can be used on fresh or fixed tissue samples and is primarily designed to image bone rather than soft tissues. It requires complex back-projection algorithms to produce a two-dimensional image, and it is an expensive instrument. sTSLIM requires that a specimen be chemically fixed, decalcified, and cleared; but it produces high-resolution images of soft and bony tissues with minimum image postprocessing and is less expensive than microCT. In this article, we discuss the merits and disadvantages of each method individually and when combined.
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