Title
MicroFilament Analyzer identifies actin network organizations in epidermal cells of **Arabidopsis thaliana** roots
Author
Faculty/Department
Faculty of Sciences. Biology
Faculty of Sciences. Physics
Publication type
article
Publication
Subject
Biology
Source (journal)
Plant signaling & behavior
Volume/pages
8(2013) :7
ISSN
1559-2316
Article Reference
e24821
Carrier
E-only publicatie
Target language
English (eng)
Affiliation
University of Antwerp
Abstract
The plant cytoskeleton plays a crucial role in the cells' growth and development during different developmental stages and it undergoes many rearrangements. In order to describe the arrangements of the F-actin cytoskeleton in root epidermal cells of Arabidopsis thaliana, the recently developed software MicroFilament Analyzer (MFA) was exploited. This software enables high-throughput identification and quantification of the orientation of filamentous structures on digital images in a highly standardized and fast way. Using confocal microscopy and transgenic GFP-FABD2-GFP plants the actin cytoskeleton was visualized in the root epidermis. MFA analysis revealed that during the early stages of cell development F-actin is organized in a mainly random pattern. As the cells grow, they preferentially adopt a longitudinal organization, a pattern that is also preserved in the largest cells. In the evolution from young to old cells, an approximately even distribution of transverse, oblique or combined orientations is always present besides the switch from random to a longitudinal oriented actin cytoskeleton.
Handle