Publication
Title
Isolation and characterization of ten polymorphic microsatellite loci in **Ixodes arboricola**, and cross-amplification in three other **Ixodes** species
Author
Abstract
We characterized ten polymorphic microsatellite loci from the tree-hole tick, Ixodes arboricola. Loci were screened in 1118 individuals from three Belgian populations and five to ten alleles were found at each locus. Seven loci did not show deviations from HardyWeinberg equilibrium conditions and there were no indications for null alleles at these loci. The three other loci showed significant heterozygote deficiencies in at least one population, and a high potential for the occurrence of null alleles. We observed no effect of potential host DNA on the scoring of the microsatellites. Cross-amplification of the microsatellites was tested in eight specimens of three congeneric species: I. ricinus, I. hexagonus and I. frontalis. Depending on the species, six or seven of the loci were amplified in ≥4 of the 8 specimens and were polymorphic in each of these species (except for Ixaf 11 in I. frontalis and I. ricinus). These loci thus provide a tool for population genetic analysis of I. arboricola. The suitability of these markers needs to be further investigated in its congeners.
Language
English
Source (journal)
Experimental and applied acarology / Great Britain. Ministry of Agriculture Fisheries and Food Agricultural Development and Advisory Service Yorkshire and Lancashire Region. - London
Publication
London : 2013
ISSN
0168-8162
Volume/pages
61:3(2013), p. 327-336
ISI
000324833200005
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 07.05.2013
Last edited 06.07.2017
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