Isolation and characterization of ten polymorphic microsatellite loci in **Ixodes arboricola**, and cross-amplification in three other **Ixodes** species
Faculty of Sciences. Biology
Experimental and applied acarology / Great Britain. Ministry of Agriculture Fisheries and Food Agricultural Development and Advisory Service Yorkshire and Lancashire Region. - London
, p. 327-336
University of Antwerp
We characterized ten polymorphic microsatellite loci from the tree-hole tick, Ixodes arboricola. Loci were screened in 1118 individuals from three Belgian populations and five to ten alleles were found at each locus. Seven loci did not show deviations from HardyWeinberg equilibrium conditions and there were no indications for null alleles at these loci. The three other loci showed significant heterozygote deficiencies in at least one population, and a high potential for the occurrence of null alleles. We observed no effect of potential host DNA on the scoring of the microsatellites. Cross-amplification of the microsatellites was tested in eight specimens of three congeneric species: I. ricinus, I. hexagonus and I. frontalis. Depending on the species, six or seven of the loci were amplified in ≥4 of the 8 specimens and were polymorphic in each of these species (except for Ixaf 11 in I. frontalis and I. ricinus). These loci thus provide a tool for population genetic analysis of I. arboricola. The suitability of these markers needs to be further investigated in its congeners.