Publication
Title
A LiTat 1.5 variant surface glycoprotein-derived peptide with diagnostic potential for Trypanosoma brucei gambiense
Author
Abstract
Objective To evaluate the accuracy of a peptide, corresponding to the variant surface glycoprotein (VSG) LiTat 1.5 amino acid (AA) sequence 268281 and identified through alignment of monoclonal antibody selected mimotopes, for diagnosis of Trypanosoma brucei gambiense sleeping sickness. Methods A synthetic biotinylated peptide (peptide 1.5/268281), native VSG LiTat 1.3 and VSG LiTat 1.5 were tested in an indirect ELISA with 102 sera from patients with HAT and 102 endemic HAT-negative controls. Results The area under the curve (AUC) of peptide 1.5/268281 was 0.954 (95% confidence interval 0.9180.980), indicating diagnostic potential. The areas under the curve of VSG LiTat 1.3 and LiTat 1.5 were 1.000 (0.9821.000) and 0.997 (0.9731.000), respectively, and significantly higher than the AUC of peptide 1.5/268281. On a model of VSG LiTat 1.5, peptide 1.5/268281 was mapped near the top of the VSG. Conclusions A biotinylated peptide corresponding to AA 268281 of VSG LiTat 1.5 may replace the native VSG in serodiagnostic tests, but the diagnostic accuracy is lower than for the full-length native VSG LiTat 1.3 and VSG LiTat 1.5. Objectif Evaluer la precision diagnostique d'un peptide correspondant a une variante de la sequence des acides amines 268 a 281 de la glycoproteine de surface LiTat 1.5 et identifie grace a l'alignement des mimotopes selectionnes des anticorps monoclonaux, pour le diagnostic de la maladie du sommeil due a Trypanosoma brucei gambiense. Methodes Un peptide synthetique biotinyle (peptide 1.5/268-281) et les proteines de surface natives LiTat 1.3 et LiTat 1.5 ont ete testes dans une reaction ELISA indirecte sur 102 echantillons de serum provenant de patients atteints de THA et 102 controles negatifs de zone endemique. Resultats L'aire sous la courbe (AUC) du peptide 1.5/268-281 etait de 0.954 (IC95%: 0.918 a 0.980), ce qui indique un potentiel diagnostique. Les aires sous la courbe de LiTat 1.3 et LiTat 1.5 etaient de 1000 (0.982 a 1000) et 0.997 (0.973 a 1000), respectivement, et nettement superieures a l'AUC du peptide 1.5/268-281. Sur un modele de LiTat 1.5, le peptide 1.5/268-281 a ete cartographie pres du sommet de la glycoproteine de surface. Conclusions Un peptide biotinyle correspondant a la sequence des acides amines 268 a 281 de LiTat 1.5 pourrait remplacer la glycoproteine de surface native dans les tests de serodiagnostic, mais la precision du diagnostic est plus faible qu'avec l'entierete des glycoproteines de surfaces natives LiTat 1.3 et LiTat 1.5. Objetivo Evaluar la precision diagnostica de un peptido correspondiente a la secuencia de aminoacidos 268281 de la glicoproteina variante de superficie (VSG) LiTat 1.5, identificados mediante un alineamiento de una seleccion de mimotopes de anticuerpos monoclonales, para el diagnostico de la enfermedad del sueno por Trypanosoma brucei gambiense (HAT). Metodos Utilizando un ELISA indirecto, se probaron el peptido sintetico biotinilado (peptido 1.5/268-281), la VSG nativa LiTat 1.3 y la VSG LiTat 1.5, con 102 sueros de pacientes con HAT y 102 controles negativos endemicos. Resultados El area bajo la curva (ABC) del peptido 1.5/268-281 era de 0.954 (IC 95% 0.9180.980), indicando su potencial diagnostico. Las ABC de VSG LiTat 1.3 y LiTat 1.5 eran 1.000 (0.982-1.000) y 0.997 (0.9731.000) respectivamente, y significativamente mayor que el ABC del peptido 1.5/268-281. En un modelo del VSG LiTat 1.5, el peptido 1.5/268-281 se mapeo cerca de la parte superior del VSG. Conclusiones Un peptido biotinilado, que corresponde al AA 268281 del VSG LiTat 1. 5, podria reemplazar las pruebas serodiagnosticas basadas en la VSG nativa, pero la precision diagnostica es menor que para la VSG LiTat 1.3 nativa de tamano completo y la VSG LiTat 1.5.
Language
English
Source (journal)
Tropical medicine and international health. - Oxford
Publication
Oxford : 2013
ISSN
1360-2276
Volume/pages
18:4(2013), p. 461-465
ISI
000316627200011
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 05.06.2013
Last edited 05.12.2017
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