Title
Saponin production is not qualitatively changed upon callus regeneration in the medicinal shrub **Maesa perlarius**
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Publication type
article
Publication
The Hague ,
Subject
Biology
Source (journal)
Plant growth regulation. - The Hague
Volume/pages
70(2013) :1 , p. 39-48
ISSN
0167-6903
ISI
000317970800004
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Maesa perlarius is a medicinal plant that produces maesabalides, which possess selective and strong anti-leishmania activity. In this study, M. perlarius plants were regenerated from leaf-derived calli. Shoots were induced in Murashige and Skoog medium in the presence of thidiazuron in combination with alpha-naphthalene acetic acid. In contrast to seed-derived plants, callus-derived regenerants were tetraploid showing typical characteristics of higher ploidy phenotypes. We assessed the impact of indirect plant regeneration and associated increase in ploidy on the production of saponin by means of LC-MS analysis. Tetraploid M. perlarius produce a saponin profile, which was not significantly different from seed grown plants. Based on this study, we concluded that saponin production in M. perlarius is not qualitatively changed by a genome-doubling event.
E-info
https://repository.uantwerpen.be/docman/iruaauth/60de1e/2b43679.pdf
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Handle