Title
Analysis of the formalin-fixed paraffin-embedded tissue proteome : pitfalls, challenges, and future prospectives Analysis of the formalin-fixed paraffin-embedded tissue proteome : pitfalls, challenges, and future prospectives
Author
Faculty/Department
Faculty of Sciences. Chemistry
Publication type
article
Publication
Wien ,
Subject
Chemistry
Biology
Source (journal)
Amino acids. - Wien
Volume/pages
45(2013) :2 , p. 205-218
ISSN
0939-4451
0939-4451
ISI
000321947700001
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Formalin-fixed paraffin-embedded (FFPE) tissues are a real treasure for retrospective analysis considering the amount of samples present in hospital archives, combined with pathological, clinical, and outcome information available for every sample. Although unlocking the proteome of these tissues is still a challenge, new approaches are being developed. In this review, we summarize the different mass spectrometry platforms that are used in human clinical studies to unravel the FFPE proteome. The different ways of extracting crosslinked proteins and the analytical strategies are pointed out. Also, the pitfalls and challenges concerning the quality of FFPE proteomic approaches are depicted. We also evaluated the potential of these analytical methods for future clinical FFPE proteomics applications.
E-info
https://repository.uantwerpen.be/docman/iruaauth/c55fb0/a7b5340.pdf
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