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Title
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A membrane microcontactor as a tool for integrated sample preparation
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Author
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Abstract
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| A membrane microcontactor suitable to perform liquid-liquid extraction as well as evaporation in order to conduct enrichment steps in sample preparation of organ samples has been designed, fabricated, and characterized. Spacers of 100- or 200-μm high were constructed in a metal substrate with a channel width of 13 mm and the extraction kinetics in these channels was evaluated. The spacers were designed such that at the entrance and exit region a uniform flow distribution could take place and that a uniform flow profile could be guaranteed along the channel, hence allowing a large freedom in sample volume to be processed. The extraction and evaporation kinetic behavior of the device was first evaluated by extraction of a drug candidate (4-(2,5-dimethyl-pyrrol-1-y1)-2-hydroxybenzoic acid). To evaluate the device under more challenging working conditions, a homogenized mice kidney sample containing the drug candidate that was administered in life condition was cleaned and enriched with the extraction and evaporation modules and characterized by high-performance liquid chromatography, yielding an overall analysis time of 15-20 min per sample only. The system has the potential to be operated in a continuous fashion, making it appealing to be implemented in screening or high-throughput applications. |
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Language
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English
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Source (journal)
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Journal of separation science. - Weinheim, 2001, currens
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Publication
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Weinheim
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2012
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ISSN
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1615-9306
[print]
1615-9314
[online]
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DOI
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10.1002/JSSC.201200477
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Volume/pages
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35
:18
(2012)
, p. 2407-2413
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ISI
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000309059700007
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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