Title
Observations on the viability of C6-glioma cells after sonoporation with low-intensity ultrasound and microbubbles Observations on the viability of C6-glioma cells after sonoporation with low-intensity ultrasound and microbubbles
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
New York ,
Subject
Physics
Source (journal)
IEEE transactions on ultrasonics, ferroelectrics and frequency control / Institute of Electrical and Electronics Engineers [New York, N.Y.] - New York
Volume/pages
60(2013) :1 , p. 34-45
ISSN
0885-3010
ISI
000314369700004
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Ultrasound (US) and microbubbles can be used to facilitate cellular uptake of drugs through a cavitation-induced enhancement of cell membrane permeability. The mechanism is, however, still incompletely understood. A direct contact between microbubbles and cell membrane is thought to be essential to create membrane perturbations lasting from seconds to minutes after US exposure of the cells. A recent study showed that the effect may even last up to 8 h after cavitation (with residual permeability up to 24 h after cavitation). In view of possible membrane damage, the purpose of this study was to further investigate the evolution of cell viability in the range of the 24-h temporal window. Furthermore, a description of the functional changes in tumor cells after US exposure was initiated to obtain a better understanding of the mechanism of membrane perturbation after sonication with microbubbles. Our results suggest that US does not reduce cell viability up to 24 h post-exposure. However, a perturbation of the entire cell population exposed to US was observed in terms of enzymatic activity and characteristics of the mitochondrial membrane. Furthermore, we demonstrated that US cavitation induces a transient loss of cell membrane asymmetry, resulting in phosphatidylserine exposure in the outer leaflet of the cell membrane.
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