Title
No novel coronaviruses identified in a large collection of human nasopharyngeal specimens using family-wide CODEHOP-based primers No novel coronaviruses identified in a large collection of human nasopharyngeal specimens using family-wide CODEHOP-based primers
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Wien ,
Subject
Human medicine
Source (journal)
Archives of virology. - Wien
Volume/pages
158(2013) :1 , p. 251-255
ISSN
0304-8608
ISI
000313294200032
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Novel viruses might be responsible for numerous disease cases with unknown etiology. In this study, we screened 1800 nasopharyngeal samples from adult outpatients with respiratory disease symptoms and healthy individuals. We employed a reverse transcription (RT)-PCR assay and CODEHOP-based primers (CT12-mCODEHOP) previously developed to recognize known and unknown corona- and toroviruses. The CT12-mCODEHOP assay detected 42.0 % (29/69) of samples positive for human coronaviruses (HCoV), including HCoV-229 (1/16), HCoV-NL63 (9/17), and HCoV-OC43 (19/36), and additionally HCoV-HKU1 (3), which was not targeted by the diagnostic real-time PCR assays. No other coronaviruses were identified in the analyzed samples.
E-info
https://repository.uantwerpen.be/docman/iruaauth/0115ee/7f85056.pdf
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