Title
A graphene oxide amplification platform tagged with tyrosinase-zinc oxide quantum dot hybrids for the electrochemical sensing of hydroxylated polychlorobiphenyls A graphene oxide amplification platform tagged with tyrosinase-zinc oxide quantum dot hybrids for the electrochemical sensing of hydroxylated polychlorobiphenyls
Author
Faculty/Department
Faculty of Sciences. Chemistry
Publication type
article
Publication
Lausanne ,
Subject
Physics
Chemistry
Source (journal)
Sensors and actuators : B : chemical. - Lausanne
Volume/pages
190(2014) , p. 612-620
ISSN
0925-4005
ISI
000326687700082
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Graphene oxide can act as an amplification platform for the immobilization of a hybrid structure composed of tyrosinase (Tyr) and zinc oxide quantum dots (ZnO QDs). This article describes how this platform increases the sensitivity for the detection of hydroxylated polychlorobiphenyls (OH-PCBs). The adsorption of Tyr (with low isoelectric point) on the positively charged surface of ZnO QDs is based on electrostatic interactions. The scanning electron microscopic images and UVvis spectroscopic analysis demonstrated the adsorption of Tyr on ZnO QDs. The stepwise assembly process of the fabricated biosensor was characterized by cyclic voltammetry and electrochemical impedance spectroscopy. The synthesized ZnO QDs and graphene oxide were characterized by Raman spectroscopy, infrared spectroscopy, X-ray diffraction and scanning electron microscopic techniques. The determination of OH-PCBs was carried out by using square wave voltammetry over the concentration range of 2.827.65 μM with a detection limit of 0.15 μM with good reproducibility, selectivity and acceptable stability. The high value of surface coverage of ZnO QDs and small value of MichaelisMenten constant (View the MathML source) confirmed an excellent loading of the Tyr and a high affinity of the biosensor toward the detection of OH-PCBs. This biosensor and the described sensing platform offer a great potential for rapid, cost-effective and on-field analysis of OH-PCBs.
E-info
https://repository.uantwerpen.be/docman/iruaauth/ab94c6/e9124666fe0.pdf
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