Title
Quantification of circulating <tex>$CD34^{+}/KDR^{+}/CD45^{dim}$</tex> endothelial progenitor cells : analytical considerations Quantification of circulating <tex>$CD34^{+}/KDR^{+}/CD45^{dim}$</tex> endothelial progenitor cells : analytical considerations
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Amsterdam ,
Subject
Human medicine
Source (journal)
International journal of cardiology. - Amsterdam, 1981, currens
Volume/pages
167(2013) :5 , p. 1688-1695
ISSN
0167-5273
ISI
000323569600021
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The discovery of peripheral circulating cells that contribute to vasculogenesis and endothelial repair was one of the most fascinating breakthroughs in the domain of vascular research during the last two decades. The population of vasculogenic cells however, is heterogeneous and can be analyzed using different approaches including in vitro culture and flow cytometry. Circulating CD34(+)/KDR+/CD45(dim) endothelial progenitor cells (EPC) have a great potential as biomarkers in various cardiovascular diseases. With the expanding interest in this field, the development of standardized protocols is critical. In this review we describe in detail the pre-analytical and analytical factors that should be taken into account when quantifying CD34(+)/KDR+/CD45(dim) EPC using flow cytometry. Moreover, technical suggestions in order to enhance accuracy and reproducibility of this enumeration are provided. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
E-info
https://repository.uantwerpen.be/docman/iruaauth/93cc4e/d565478.pdf
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